池养鲥鱼全人工繁殖的机理

The mechanism and regulation of artificial propagation of pond-domesticated Reeves shad

本研究首次成功地在盐度０．５～１２的咸淡水池塘中驯养鲥幼鱼至６龄，并提出池养鲥鱼全人工繁殖机理。采用外源内分泌药理学和生态学的方法刺激鲥鱼早春脂肪积累和血清中的１７β－Ｅ２的合成与分泌，启动性腺发育和诱导成熟，４、５、６龄雌鱼的成熟率分别达８３．３％、８７．５％和１００％；在催产过程中采用神经介质抑制剂和可的松对抗剂，克服池养鲥鱼排卵机制障碍和产卵前紧张反应的副效应，获得约８００００粒的受精卵，受精率约７５％，从而突破了池养鲥鱼不育症。

Juvenile Reeves shad is domesticated to 6 year old for the first time in confined brackish water pond with salinity of 0.5～12. With the ectrinsic pharmacological and ecological manipulations, its gonad is induced to develop and mature, the fat accumulates in the fish body and 17 β-E 2 forms and then is secreted. The induced maturities of 4, 5, 6 year old females reach 83.3%, 87.5% and 100% respectively, while controls remain immature. The obstacles to ovulation mechanism and handling-induced stress are overcomed in the process of artificially induced spawning through physiological and ecological control. About 80 000 fertilized ova are obtained and the fertilization rate reaches 75%.