草苁蓉提取物对阿尔茨海默病大鼠乙酰胆碱及学习记忆能力的影响

Influence of Boschniakia rossica extract on the content of Ach and the capability of learning and memory in Alzheimer's disease rats

目的:探索中药草苁蓉对β淀粉样蛋白(β-AP)所致AD模型大鼠的脑内乙酰胆碱(Ach)含量及学习记忆能力的影响。方法:将60只健康SD大鼠随机分为正常组、假手术组、模型组和中药高、中、低剂量组。采用脑立体定位仪双侧Meynert核注射Aβ1-40建立AD模型,中药组予以草苁蓉提取物按高、中、低3种不同剂量灌胃治疗,于治疗后检测各组大鼠的学习记忆能力及海马、皮质部位Ach的含量。结果:较模型组,中药组大鼠学习记忆能力及Ach含量有明显提高(P<0.01)。结论:中药草苁蓉能增强AD模型大鼠海马及皮质部位Ach的含量,提高AD大鼠的学习记忆能力。

OBJECTIVE To investigate the impact of Boschniakia rossica extract on the content of Ach and learning and memory of the β-amyloid protein（β-AP）-induced model rats. METHODS 60 healthy SD rats were randomly divided into normal group, sham group, the model group and the group of Traditional Chinese Medicine with high-dose, medium-dose and low-dose. Adapting Aβ1-40 to establish the AD model, the Traditional Chinese Medicine groups were given orally with three different dose （high, medium and low） of Boschniakia rossica extract. After the treatment, the learning, memory capability and the content of Ach in the hippocampus, and cortical parts of the rats were measured. RESULTS Compared with the model group,traditional Chinese Medicine group were learning and memory ability and the content of Ach in the enhance significantly （P〈0.0）1）. CONCLUSION The Boschniakia rossica extract can en hance the content of Ach and improve the learning and memory capability of AD rats.