红花黄色素对家兔缺血再灌注心肌的保护作用

Protections of Safflor Yellow on the myocardium in rabbits with acute myocardial ischemia and reperfusion

目的:通过在体兔心肌缺血再灌注模型,观察红花黄色素对家兔缺血再灌注心肌是否具有保护作用。方法:24只新西兰家兔随机分为缺血再灌注组(IR组)、缺血预处理组(IPC组)及红花黄色素组(SY组),每组8只。分别于结扎前、再灌注3h、再灌注6h采兔血,测定各组血超氧化物歧化酶(SOD)、丙二醛(MDA)、肌酸激酶同工酶(CK-MB)和肌钙蛋白I(cTNI)的水平。实验结束,用氯化硝基四氮唑蓝(NBT)染色测定心肌梗死面积。结果:与IR组相比,SY组和IPC组在再灌注各时间点MDA、CK-MB、cTNI浓度均降低(P<0.05),而SOD升高(P<0.05);SY组和IPC组坏死面积占总面积之比小于IR组(P<0.05)。结论:红花黄色素和缺血预处理一样能减轻缺血再灌注损伤。

OBJECTIVE To study whether Safflor Yellow（SY） produces cardioprotection on rabbit cardiac ischemic-reperfusion model in vivo. METHODS 24 Rabbits were randomly distributed to three group（n = 8 in each group） ：①Ischemia-reperfusion（IR） ;②Ischemic preconditioning（IPC） ;③Safflor Yellow （SY） . Each group was determined the density of serum superoxide dismutases（SOD）, malonaldehyde （MDA）, creatinkinase isoenzyme （CK-MB） and troponin Ⅰ （cTNⅠ） at three times： before LADO, reperfusion 3h and reperfusion 6h. The infarction sizes（IS） were measured with nitroblue tetrazolium（NBT） dye. RESULTS Compared with IR group, the levels of MDA ,CK-MB and cTNⅠ in SY group and IPC group were reduced significantly （P〈0. 05） at reperfusion 3h and reperfusion 6h, on the contrary, the levels of SOD was increased （P〈0. 05） ;The IS （percent age necrosis/total areas） was reduced significantly （P〈0. 05） in SY group and IPC group. CONCLUSION Safflor Yellow as same as ischemic preconditioning can protect the ischemia damage of myocardium.