蒙古族人群肺癌易感性与GSTM1基因多态性

Relationship of GSTM1 polymorphism and lung cancer susceptibility in Mongolian population

目的研究内蒙古地区蒙、汉族人群谷胱甘肽硫转移酶M1基因（GSTM1）多态性,探讨蒙古族人群肺癌易感性与GSTM1基因多态性关系。方法采用多重PCR等方法对325名健康汉族人、310名健康蒙古族人及304例蒙古族肺癌患者进行GSTM1基因多态性检测,分析GSTM1基因型的分布频率,并探讨GSTM1基因多态性与蒙古族人群肺癌易感性的关系。结果健康蒙、汉族人群GSTM1基因型分布频率分别为：GSTM1功能型（＋）61.61%,GSTM1缺陷型（-）38.39%和GSTM1（＋）57.23%,GSTM1（-）42.77%;蒙古族肺癌组与对照组GSTM1（＋）和GSTM1（-）的分布频率分别为52.96%,47.04%和61.61%,38.39%;携带GSTM1（-）的个体患肺癌的风险增高,OR值为1.43（95%CI=1.03-1.96,P〈0.05）。结论GSTM1基因型分布频率在内蒙古地区蒙、汉族人群间比较无明显差别;GSTM1（-）是蒙古族人群肺癌的易感因素。

Objective To study the relationship of glutathione s-transferase M1（GSTM1） polymorphism and lung cancer susceptibility in a Mongolian population.Methods The multiplex polymerase chain reaction were used to analyze the genetic polymorphism of GSTM1 among 325 healthy Han nationality,310 healthy Mongolian nationality,and 304 Mongolian lung cancer patients.The relationship between the polymorphism of GSTM1 and susceptibility of lung cancer in the Mongolian population was studied.Results The frequency of GSTM1 of healthy Mongolian was 61.61% for GSTM1（＋）,38.39% for GSTM1（-） and that of Han was 57.23% for GSTM1（＋）,42.77% for GSTM1（-）.Chi-square tests showed that there was no significant difference between the two guoups（P〈0.05）.The fequency of GSTM1（＋） and GSTM1（-） of lung cancer guoup and healthy Mongolian guoup were 52.96%,47.04% and 61.61%,38.39%,respectively.The Chi-square tests showed that there was a significant difference between the two guoups.The people carriying with GSTM1（-） genotype had a 1.43 folds increased risk of lung cancer than those carrying with GSTM1（＋） with a 95%CI of 1.03-1.96.Conclusion There is no significant difference in the frequency of GSTM1 gene between the healthy Mongolian and Han Nationality.GSTM1（-） is a susecptibility factor of lung cancer for Mongolian Nationality in Inner Mongolia.