期刊文献+

牛奶中醛脱氢酶的动力学

KINETIC STUDY OF ALDEHYDE DEHYDROGENASE IN MILK
下载PDF
导出
摘要 以新鲜牛奶作为研究对象,利用分光光度计,基于底物浓度、温度和pH值研究以乙醛为底物的乙醛脱氢酶动力学系统。结果表明,牛奶中的醛脱氢酶酶促反应动力学符合米氏方程所描述的单底物酶促反应动力学,相应的动力学参数Km=0.0063mol/L,Vmax=0.036U/min。此外,醛脱氢酶不是耐热酶,它的最适温度在35℃左右,最适pH在7.0左右,底物浓度与酶活力的关系基本符合酶促反应动力学。同时研究了10种试剂对醛脱氢酶的抑制或激活作用,硝酸钙和硫脲是比较理想的抑制剂,硝酸钾和钼酸铵则是比较理想的激活剂。 On the substrate concentration, temperature and pH, the acetaldehyde dehydrogenase Based kinetics in fresh milk was investigated by a spectrophotometer with aldehyde being substrate. The results showed that the acetaldehyde dehydrogenase kinetics was consistent with the enzyme kinetics of a single substrate described by Michaelis-Menten. Its parameters Km is 0.006 3 mol/L and Vmax is 0.036 U/min. In addition, the enzyme is not heat-resistant. Its optimum temperature and pH is around 35 ℃ and 7.0, respectively. Overall, the relationship between the substrate concentration and enzyme activity corresponds to the enzyme kinetics.At the same time have studied inhibition and activator acting to aldehyde dehydrogenase. Ca(NO3)2 and liuniao are ideal Inhibitor. KNO3 and ammonium molybdate are ideal Activator.
出处 《食品研究与开发》 CAS 北大核心 2009年第12期54-57,共4页 Food Research and Development
关键词 牛奶 醛脱氢酶 动力学 抑制剂 激活剂 milk aldehyde dehydrogenase kinetics inhibitor activator
  • 相关文献

参考文献6

二级参考文献20

  • 1Cappola TP, Kass DA, Nelson GS, et al. Allopurinol improves myocardial efficiency in patients with idiopathic dilated candiomyopathy[J]. Circulation, 2001,4: 2407-2411.
  • 2Cai H, Harrison DG. Endothelial dysfunction in cardiovascular disease: the role of oxidant stress[J]. Circ Res,2000,87:840-844.
  • 3Houston M, Chumley P, Radi R, et al. Xanthine oxidase reaction with nitric oxide and peroxynitrite[J]. Arch Biochem Biophys, 1998,355: 1-8.
  • 4Guthikonda S, Sinkey C, Barenz T, et al. Xanthine oxidase inhibition reverses endothelial dysfunction in heavy smokers[J]. Circulation,2003, 107: 416-421.
  • 5Ohara Y, Peterson TE, Harrison DG. Hypercholesterolemia increases endothelial superoxide anion production[J] .J Clin Invest, 1993, 91:2546-2551.
  • 6Landmesser U, Spiekermann S, Dikalov S, et al. Vascular oxidative stress and endothelial dysfunction in patients with chronic heart failure: role of xanthine-oxidase and extracellular superoxide dismutase[J]. Circulation ,2002,106:3073-3078.
  • 7Patetsios P, Song M, Shutze WP, et al. Identification of uric acid and xanthine oxidase in atherosclerotic plaque [ J ]. Am J Cardiol ,2001, 88:188-191.
  • 8Swei A, Lacy F, DeLano FA, et al. A mechanism of oxygen free radical production in the Dahl hypertensive rat[J]. Microcirc, 1999,6:179-187.
  • 9Laakso J, Mervaala E, Himberg J, et al. Increased kidney xanthine oxidoreductase activity in salt-induced experimental hypertension[J].Hypertension, 1998,32: 902-906.
  • 10Mazzali M, Hughes J, Kim Y, et al. Elevated uric acid increases blood pressure in the rat by a novel crystal independent mechanism[J]. Hypertension ,2001,38: 1101-1106.

共引文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部