摘要
在乙醇介质中,λex/λem=280/495nm由于强荧光染料吖啶黄的荧光与苏丹红I的吸收光的等色性,苏丹红I能够有效吸收吖啶黄发出的荧光,使其荧光猝灭,依此建立荧光测定苏丹红I的新方法,该方法快速简便其线性范围为:1.5×10-6mol/L～3.5×10-5mol/L。用于辣椒、辣椒酱食品中痕量苏丹红I的测定,相对标准偏差为:0.8%～2.7%,回收率为:89.5%～109.0%,方法检出限为:6.4×10-7mol/L。
Depending on the isochromatism between the fluorescence of Acridine Yellow and the absorption of Sudan Ⅰ, at λex/em=280/495 nm, Sudan Ⅰ absorbed the fluorescence of Acridine Yellow effectively in the medium of ethanol, which made the fluorescence of Acridine Yellow quenched, so we built the new way to determine the concentration of Sudan I. The range of determining concentration of Sudan I was 1.5×10^-6 mol/L- 3.5×10^-5 mol/L. The measurement has been applied to the determination of Sudan I in capsicum powder and chili sauce. The relative standard deviation was 0.8 %-2.7 % for 6 parallel determination of Sudan Ⅰ samples and the recovery of Sudan I was 89.5 %-109.0 %. The detection limit of the method was up to 6.4×10^-7 mol/L.
出处
《食品研究与开发》
CAS
北大核心
2009年第12期131-133,共3页
Food Research and Development
关键词
荧光猝灭
苏丹红
吖啶黄
含量
fluorescence quenching
Sudan Ⅰ
acridine yellow
concentration
