用发根农杆菌(Agrobacterium rhizogenes)转化大豆的研究Ⅰ.大豆毛状根的诱导

STUDY ON THE SOYBEAN TRANSFORMEDBY AGROBACTERIUM RHIZOGENESⅠ. Induction of hairy root on the soybean

本实验用发根农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ）对大豆属的三个种：野生大豆（Ｇｌｙｃｉｎｅｓｏｊａ），半野生大豆（Ｇｌｙｃｉｎｅｇｒａｃｉｌｉｓ）和栽培大豆（Ｇｌｙｃｉｎｅｍａｘｌ）的不同外植体进行感染诱导毛状根，在无菌苗靠近子叶节部位诱导出毛状根．冠瘿碱（ｏｐｉｎｅ）检测表明；由感染发根农杆菌诱导的毛状根中有甘露碱（ｍａｎｎｏｐｉｎｅ）的存在．说明Ｒｉ质粒的Ｔ－ＤＮＡ已转移到大豆的转化根中．

Root were only induced at cotyledons node of mature embryos in various explants of Glycine soja. Glycine gracillis, Glycine max with Agrobacterium rhizogenes. Inspection of opine showed that theres was mannopine in the hairy root iduced by A. rhizogenes, and therefore that T-DNA of Ri plasmid was successfully transfered.