4种鳗鲡同工酶的比较研究

A comparative study on sozymes in Four Species of Anguilla

以日本鳗鲡(Anguilla japonica)、澳洲鳗鲡(A.australis)、欧洲鳗鲡(A.anguilla)和美洲鳗鲡(A.rostrata)成体为试验材料,应用垂直聚丙烯酰胺凝胶电泳方法,对4种鳗鲡的眼球、肌肉、肝脏、心脏和肾脏5种组织的8种同工酶(酯酶EST、乳酸脱氢酶LDH、苹果酸脱氢酶MDH、葡萄糖脱氢酶GCDH、葡萄糖-6-磷酸脱氢酶G6PD、醇脱氢酶ADH、超氧化物歧化酶SOD和谷氨酸脱氢酶GDH)进行比较研究。结果表明:GDH在5种组织中均没有表达,7种同工酶(EST、LDH、MDH、GCDH、G6PD、ADH、SOD)在4种鳗鲡均表现出很强的组织特异性。4种鳗鲡的同工酶谱表型非常相似,在同一组织中同工酶的存在位点、表达形态、活性等方面是类似的,但也表现出一定的种间差异性。应用酶谱差异指数D对4种鳗鲡进行聚类分析,结果提示日本鳗鲡和澳洲鳗鲡有较近的亲缘关系、而欧洲鳗鲡和美洲鳗鲡有较近的亲缘关系,这与4种鳗鲡的形态特征和生物学特征相吻合。

By using the vertical polyacrylamide gel electrophoresis, eight isozymes （i. e. , EST, LDH, MDH, GC- DH, G6PD, ADH, SOD, GDH ） from five tissues （i. e. , eye, muscle, liver, heart and kidney） of four species of Anguilla （i. e. , A. japonica, A. australis, A. Anguilla and A. rostrata） were analyzed. The results showed that GDH was not detected in all samples, while EST, LDH, MDH, GCDH, G6PD, ADH and SOD exhibited apparent tissue specificities. In the four species, the isozyme electrophorectograms showed similarities in their locus numbers, allele activities in a same tissue, significant differences among the tissues, and differences from species to species. By means of UPGMA, A. japonica was found to relate more closely to A. australis, while A. anguilla to a. rostrata.