IL-1Ra对阿霉素肾病大鼠血清IL-6、IL-10、IL-13、IL-1的影响

EFFECTS OF IL-1 RECEPTOR ANTAGONIST ON SERUM IL-6,IL-10,IL-13,AND IL-1 LEVELS IN ADRIAMYCIN NEPHROSIS IN RATS

目的:探讨IL-1Ra对阿霉素肾病大鼠血清IL-6、IL-10、IL-13、IL-1的影响。方法:将大鼠随机分为正常对照组(A组,n=10)、阿霉素肾病组(B组,n=10)、阿霉素肾病IL-1Ra治疗组(C组,n=10)、阿霉素肾病生理盐水治疗组(D组,n=10),2周后检测尿24hUP、血清IL-6、IL-10、IL-13、IL-1、Al、T-ch、BUN、Scr。结果:B、C、D组血清IL-6、IL-10、IL-13水平明显高于A组(P<0.01),IL-1Ra治疗后,24hUP、T-ch较B、D组及C组IL-1Ra治疗前明显降低(P<0.01),Al、IL-6、IL-10、IL-13水平均较B、D组及C组IL-1Ra治疗前增高(P<0.05),IL-1血清水平较B组、D组、C组IL-1Ra治疗前明显降低(均P<0.01),接近A组水平(P>0.05)。结论:IL-1Ra对阿霉素肾病大鼠有明显治疗作用的同时能提高抗炎细胞因子IL-6、IL-10、IL-13的血清水平。

Objective：To study the effects of IL-1 receptor antagonist on serum IL-6,IL-10,IL-13,and IL-1 levels in rats with adriamycin（ADR） nephrosis. Methods： The rats were randomly dividied into the control group （group A, n = 10）, the ADR nephrosis group （group B, n = 10）, the IL-1Ra on ADR nephrosis group （group C, n = 10）, the sodium chloride on ADR nephrosis group （group D, n = 10）,and the changes of the urine protein （UP）, serum IL-6, IL-10, IL-13, albumin （ A1）, total cholesterol （ TC）, urea nitrogen （UN）, and creatinine（Cr） levels were detected after two weeks. Results：The levels of serum IL-6, IL-10, and IL-13 were higher in groups B,C and D than those in group A（ P〈0. 01）. After IL-1Ra treatment, the levels of the 24h UP and TC were significantly decreased in groups B and D（ P d0.01） ,and the levels of albumin, IL-6,IL-10 and IL-13 were increased（ P 〈0. 05）. After treated with IL-1Ra,serum levels of IL-1 were significantly lower in ADR group than in groups B, D and before treated with IL-1Ra groups （ P〈 0.01）, compared with control group, there was not significant difference （ P 〉0.05）. Conclusion： The results suggest a significant therapeutic effect of IL-1Ra on the ADR nephrosis,and elevates the levels of serum anti-inflammatory cytokine.