摘要
目的:探讨IL-1Ra对阿霉素肾病大鼠血清IL-6、IL-10、IL-13、IL-1的影响。方法:将大鼠随机分为正常对照组(A组,n=10)、阿霉素肾病组(B组,n=10)、阿霉素肾病IL-1Ra治疗组(C组,n=10)、阿霉素肾病生理盐水治疗组(D组,n=10),2周后检测尿24hUP、血清IL-6、IL-10、IL-13、IL-1、Al、T-ch、BUN、Scr。结果:B、C、D组血清IL-6、IL-10、IL-13水平明显高于A组(P<0.01),IL-1Ra治疗后,24hUP、T-ch较B、D组及C组IL-1Ra治疗前明显降低(P<0.01),Al、IL-6、IL-10、IL-13水平均较B、D组及C组IL-1Ra治疗前增高(P<0.05),IL-1血清水平较B组、D组、C组IL-1Ra治疗前明显降低(均P<0.01),接近A组水平(P>0.05)。结论:IL-1Ra对阿霉素肾病大鼠有明显治疗作用的同时能提高抗炎细胞因子IL-6、IL-10、IL-13的血清水平。
Objective:To study the effects of IL-1 receptor antagonist on serum IL-6,IL-10,IL-13,and IL-1 levels in rats with adriamycin(ADR) nephrosis. Methods: The rats were randomly dividied into the control group (group A, n = 10), the ADR nephrosis group (group B, n = 10), the IL-1Ra on ADR nephrosis group (group C, n = 10), the sodium chloride on ADR nephrosis group (group D, n = 10),and the changes of the urine protein (UP), serum IL-6, IL-10, IL-13, albumin ( A1), total cholesterol ( TC), urea nitrogen (UN), and creatinine(Cr) levels were detected after two weeks. Results:The levels of serum IL-6, IL-10, and IL-13 were higher in groups B,C and D than those in group A( P〈0. 01). After IL-1Ra treatment, the levels of the 24h UP and TC were significantly decreased in groups B and D( P d0.01) ,and the levels of albumin, IL-6,IL-10 and IL-13 were increased( P 〈0. 05). After treated with IL-1Ra,serum levels of IL-1 were significantly lower in ADR group than in groups B, D and before treated with IL-1Ra groups ( P〈 0.01), compared with control group, there was not significant difference ( P 〉0.05). Conclusion: The results suggest a significant therapeutic effect of IL-1Ra on the ADR nephrosis,and elevates the levels of serum anti-inflammatory cytokine.
出处
《广西医科大学学报》
CAS
2009年第5期660-663,共4页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.30460137)
广西自然科学基金资助项目(No.桂科自0229061)
广西科学研究与技术开发计划项目(No.桂科基01410251)
广西卫生厅科研基金资助项目(No.9955)