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急性髓系白血病细胞株APP表达及APP-siRNA对HL60细胞的沉默作用 被引量：1

STUDY ON THE EXPRESSION OF APP GENE IN AML CELL LINES AND SILENCING EFFECT OF APP-siRNA AIMED ON HL60 CELL
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摘要 目的:研究淀粉样前体蛋白(APP)基因在HL60、HL60/ADM、U937和kasumi-1细胞株中的表达及筛选针对APP基因的有效干扰片段。方法:采用荧光定量PCR和Western blot方法分别检测APP基因在4种细胞株中的mRNA和蛋白表达。用化学法合成4条针对APP的干扰片段和一条阴性对照,脂质体法转染HL60细胞,分别用定量PCR和Western blot检测APP表达。结果:在4种细胞株中,APPmRNA和蛋白表达由高到低依次为:kasumi-1、HL60、HL60/ADM和U937,kasumi-1和HL60均显著高于HL60/ADM、U937(P<0.05)。同阴性对照相比较,4条干扰片段均能不同程度地抑制APP表达,其中siRNA-4效果最佳,APPmRNA和蛋白分别下降了约64%和65%。结论:APP基因在不同AML细胞株中表达差异显著。siRNA-4的干扰效果最佳。 Objective. To study the expression of APP gene in four acute rnyeloid leukemia cell lines, kasumi- 1, HL60, HL60/ADM and U937, and to observe the silencing effect of APP-siRNA aimed on HL60 cell. Methods：The expression of APP gene was measured by real-time PCR and Western blot at the mRNA and protein level respectively. Four pairs of siRNA were chemically synthesized and transfected by lipofectamine 2000 to block the expression of APP gene in HL60 cell, and the expression of APP gene was measured by real-time PCR and Western blot. Results：The cell lines whose APP expression from high to low were kasumi-1 ,HL60,HL60/ADM, and U937, and the APP expression of kasumi-land HL60 was remarkably higher than that of HL60/ADM and U937（ P 〈0.05）. And APP siRNA -4 potently suppressed the expression of APP mRNA and protein by 64% and 65% in HL60. Conclusion：The expression of APP gene among AML cell lines was significantly different, and APP siRNA-4 might be used as the effective interfering sequences in the following experiment.
作者 王巍 孟凡义 黄走方 赵卫华
机构地区 南方医科大学南方医院血液科 广东医学院
出处 《广西医科大学学报》 CAS 2009年第5期681-684,共4页 Journal of Guangxi Medical University
基金 广东省科技厅 卫生厅联合攻关项目(No.B30202)
关键词 急性髓系白血病 APP基因 RNA干扰 acute myeloid leukemia APP RNA interference
分类号 R733 [医药卫生—肿瘤]
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参考文献9

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共引文献6

同被引文献6

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广西医科大学学报
2009年 第5期

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