标记抗体染色病毒空斑计数技术的建立

ESTABLISHMENT OF PLAQUE COUNTING TECHNIC BY LABELED ANTIBODIES STAINING

用鸡马立克氏病病毒血清１、２、３型毒株及其对应的异硫氢酸荧光素（ＦＩＴＣ）标记的型特异单克隆抗体（单抗）和型共同单抗为试验材料，以免疫荧光抗体技术（ＦＡ）为基础，并加以改进，建立了标记抗体染色病毒空斑计数技术．该技术能克服常规病毒空斑计数技术不能计数一种样品含有两种或两种以上病毒的各自空斑数的缺点，能迅速准确计数出多病毒样品中病毒各自空斑数及其空斑总数。

A new method counting virus plaque form unit (PFU) called plaque counting technic by labeled antibodies staining (PCTLAS) was established. PCTLAS was characterized by higher sensitivity, specificity, better reproducibililiy and more rapidity. Compared with traditional virus PFU counting method, PCTLAS could simultaneously count PFU of two or more serotypes or viruses strains in one specimen respectively, such as bivalent or trivalent vaccine containing two or three serotypes viruses.