大豆与花生愈伤组织的诱导与培养

Callus Culture of Soybean and Peanut

大豆品种Ｍｏｎｋｅｙ、Ｒ３４、８４－８７种子萌发４ｄ后，取其子叶、子叶节、子叶尖芽作为外植体，于５ＢＡ培养基和１ＢＡ培养基中培养，只能诱导白色愈伤组织及生根，不能获得再生植株．不同基因型的花生种子萌发需要不同时间．取不同叶龄的小叶作为外植体在Ｂ３Ｎ１培养基上诱导芽分化，后转至Ｂ５培养基上诱导芽伸长，最后于Ｎ２培养基上诱导生根，获得再生植株．芽诱导率为７９４％～９３８％．

After four days'sprouting of three strains of soybean,cotyledon,cotyledonany node and bud are used as explants and are cultured in 5BA medium and 1BA medium. But they can not produce calluses that regenerate plants They solely can be induced to produce calluses and roots It takes different time for peanut seeds of different genotypes to sprout Little leaf, as explant, is cultured in B 3N 1 medium and induced to bud's differentiation Then the explant is cultured in B 5 medium to induce bud's extending At last it is cultured in N 2 medium to induce root's appearance and plant The inducing rate of bud is 79 4%～93 8%