土壤细菌基因资源的直接分离——16S核糖体RNA基因模式

Direct Cloning of Bacterial Genes From Soil——16S Ribosomal RNA Gene as a Model

细菌遗传资源的开发利用必须先分离纯培养物，但绝大多数环境细菌无法人工培养．由于基因工程技术能直接利用基因元件，为绕过人工培养的困难，我们以细菌１６Ｓ核糖体ＲＮＡ基因为模式，建立了直接从土壤中分离基因元件的方法．该方法包括直接从土壤中分离ＤＮＡ、ＰＣＲ扩增基因和ＰＣＲ产物克隆等步骤，为直接收集、利用土壤细菌遗传资源奠定了基础．

Pure cultures are the prerequisite for utilizing bacterial genetic resources However, the major portion of environmental bacteria can not survive artificial cultivation. Since single gene element can be engineered by modern biotechniques,a direct cloning method of bacterial genes from soil was developed using 16S ribosomal RNA genes as a model in order to detour artificial cultivation This method includes DNA isolation from soil,PCR amplification of desirable genes and cloning of PCR products It has provided basis for direct collection and utilization of soil bacterial gene resources