石楠组培苗生根的影响因素研究

Effect of Several Factors on the Rooting of Photinia serrulate

[目的]寻找影响石楠组培苗生根的关键因子。[方法]首先比较生长素NAA、IBA及蔗糖不同浓度对石楠试管苗(2～3cm)生根的影响,并选用IBA和NAA2种生长素,分别设3种浓度,通过光、暗(8d)培养对比试验,研究生长素和暗培养时间对石楠组培苗生根的影响。[结果]生根培养中适当降低蔗糖的浓度、前期暗培养8d能显著提高石楠组培苗的生根率和生根数。生长素是石楠生根所必需的,培养基中同时添加NAA和IBA有利于根的分化,石楠组培苗在1/2MS+IBA0.5mg/L+NAA0.5mg/L+蔗糖20g/L培养基中生根率和生根数最高,分别为75.7%和6.9。[结论]为石楠规模化生产和转基因育种提供了理论依据。

[ Objective ] The key factor affecting the rooting of Photinia serrulate was experimented. [ Method ] The impact of the plant growth regulator （IBA and NAA with 3 concentrations, respectively） and time of dark culture on the rooting of Photinia serrulate was analyzed based on the experiment in the different concentrations of IBA,NAA and sucrose affecting the rooting of Photinia serrulate with the height of 2-3 cm. [ Results] The number and rate of its plantlet rooting could be significantly improved while the concentration of sucrose in rooting medium was appropriately reduced and the time of dark pre-culture was 8 days. The plant growth regulator is necessary for Photinia serrulate rooting and both NAA and IBA added in the medium were conducive to the root regeneration of the plant. The highest rate（75.7% ） and number（6.9 root） of Photinia serrulate rooting appeared in the culture medium： 1/2 MS ＋ IBA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ sucrose 20 g/L. [ Conclusion] The theoretical basis of large-scale production and transgenic breeding of Photinia serrulate was provided.