用低龄动物制备高效价Et免疫血清的探讨

Research on Preparation of High Titer Et Immune Serum by Low Age Animals

[目的]建立一种用低龄日本大耳白兔制备高效价迟钝爱德华氏菌(Edwardsiellatarda,Et)免疫血清的方法。[方法]先制备迟钝爱德华氏菌(Et)抗原。用日本大耳白兔作免疫动物,分2组试验,一组是2月龄组(T组),另一组是6月龄(S组)。采取耳缘静脉注射的方法,小剂量连续多次免疫日本大耳白兔,免疫后,收集免疫血清,然后用微量凝集反应法测定免疫血清抗体效价。[结果]凝集反应显示,在免疫剂量相同的情况下,第1次试血测Et免疫血清效价,S组的血清效价比T组的血清效价高,但在第2次试血测Et免疫血清效价时T组的血清效价与S组的血清效价一致。[结论]试验中所采用的T组动物(日本大耳白免)月龄较小,免疫后仍产生了与S组一致的高效价抗血清,说明用低龄日本大耳白兔制备高效价Et免疫血清的方法可行。

[ Objective ]The study aimed to establish an approach that prepared high titer Et immune serum by low age Japanese rabbits. [ Method] Antigen of Et was prepared at first, taking Japanese rabbits as immunized animal, dividing two groups of experiments, one is two- month-old group （T group）, another is six-month-old group （S group）. Using auricular intravenous method, Japanese rabbits were continuous immunized with low-dose, then collecting immune sera. Immune serum antibody titer was determined with micro-agglutination reaction method. Result] Agglutination reaction showed that the Et serum titer of S group is higher than the T in the first testing with the same dose. But in the second testing, the serum titer of the T group and S group was consistent. [ Conclusion] The animals in the T group （Japanese rabbits） have smaller months, and produced high titer antiserum was consistent with S group, which indicated that the method of preparation of high titer Et immune serum by low age Japanese rabbits was feasible.