低温胁迫下硼酸对兰引3号结缕草的生理效应初探

The Physiological Effect of Boric Acid on Zoysia japonica cv.Lanyin No 3 under Low Temperature Stress

[目的]探讨低温胁迫下不同浓度的硼酸溶液对兰引3号结缕草各项生理指标的影响,为提高低温胁迫下兰引3号结缕草草坪质量提供参考。[方法]以兰引3号结缕草为供试品种,用2种不同浓度的硼酸溶液(16、32μmol/L)喷施2次,研究低温胁迫下硼酸对兰引3号结缕草叶绿素、可溶性糖、游离脯氨酸、丙二醛含量及相对膜透性的影响。[结果]低温胁迫下16μmol/L硼酸处理及32μmol/L硼酸处理都能提高兰引3号结缕草叶绿素、可溶性糖、游离脯氨酸含量,同时降低丙二醛含量,另外,对保护细胞膜系统也起到积极作用。其中,以16μmol/L硼酸处理的效果较好,叶绿素含量、可溶性糖含量及游离脯氨酸含量分别比对照提高了37.5%、41.2%、45.1%,而丙二醛含量则降低了16.4%,相对膜透性降低了145.0%(A260)和50.0%(A280)。[结论]低温胁迫下,16μmol/L硼酸处理有利于增强兰引3号结缕草抵御低温冷害的能力。

[ Objective I The aim was to study the effect of boric acid on Zoysia japonica ev. Lanyin No 3 under low temperature stress so as to provide reference for improving lawn quality of Zoysia japonica cv. Lanyin No 3 under low temperature stress. [ Method] Two density boric acid which were 16μmol/ L and 32 μmol/L respectively were sprayed twice on Zoysia japonica cv. Lanyin No 3 to study effect of boric acid on the chlorophyll ,the soluble sugar, the dissociative proline, the malonaldehyde content and the relative membrane penetrability of Zoysia japonica cv. Lanyin No 3. [ Result] The results showed that under the low temperature stress the 16 μmol/L boric acid treatment and the 32 μmol/L boric acid treatment could enhance the chlorophyll, the soluble sugar, the dissociative proline content of Zoysia japonica steud. Lanyin No 3,simultaneously the malonaldehyde content reduced, As a result, the cell membrane system had been protected. The 16 μmoL/L boric acid treatment got the better effect,the chlorophyll content, the soluble sugar content and the dissociative proline content enhanced separately 37.5% ,41.2% ,45.1% compared to the comparison ,but the malonaldehyde content reduced 16.4% ,the relative membrane penetrability reduced 145.0% （A260） and 50.0% （A280 ）. [ Conclusion] The 16 μmol/L boric acid treatment could enhance resistance of low temperature stress of Zoysia japonica cv. Lanyin No 3.