酶水解液的丁醇发酵条件优化研究

Optimization of Butanol Production from Corn Straw Hydrolysate by Clostridium acetobutylicum

以酶水解液为发酵基质,采用Plackett-Burman设计法对影响丙酮丁醇梭菌(Clostridium acetobutylicumCICC8008)厌氧发酵生产丁醇的15个因子进行筛选。结果表明,影响该菌的厌氧发酵的主要影响因子为CaCO3。在该基础上采用响应曲面法对CaCO3浓度、温度、时间3个因子的影响进行研究,得出多元二次回归方程拟合3种因素与丁醇产量间的函数关系,并根据优化结果与试验验证,当CaCO3浓度为5.04g/L,温度为35℃,时间为70h,丁醇发酵从初始发酵培养条件下的5.15g/L提高到6.20g/L,产量提高了20%。

With enzymatic hydrolysis as the fermentation substrate, Plackett-Burman design method was adopted to screen fifteen influen- cing factors for butanol production by Clostridium acetobutylicum CICC 8008. The results showed the key factor was CaCO3. Based on these results, response surface method was used for further study on the effects of CaCO3 , temperature and reaction time in order to find out the optimum concentration levels. The concentration of butanol could reach 6.20 g/L, compared to initial 5.15 g/L, and the yield was enhanced by 20% under the conditions of CaCO3 concentration 5.04 g/L, temperature 35 ℃ , reaction time 70 h.