摘要
目的探讨全反式维甲酸(ATRA)对动脉粥样硬化(AS)模型兔动脉骨桥蛋白(OPN)表达的影响。方法复制AS兔模型,采用免疫组化和Western blot分析主动脉OPN的表达。结果成功建立AS兔模型,高脂组兔主动脉OPN蛋白表达水平增加,经ATRA治疗后OPN蛋白表达下降,AS斑块减少。结论ATRA可能通过降低动脉OPN的表达抑制AS的形成。
Objective To study the effect of alhransretinoic acid on expression of osteopontin in the artery wall of atherosclerotic rabbits. Methods The rabbit model of atherosclerosis was induced by a high-cholesterol diet. The blood lipid levels were assayed in the serum of each group. OPN expression was detected by Western blot and im- munohistoehemical methods. Results The atherosclerosis model was established successfully. After fed with cho- lesterol for twelve weeks, the expression of OPN in the artery of atherosclerotic rabbits increased markedly, whereas the expression of OPN after fed with alhransretinoic acid for twelve weeks decreased compared with that of athcro- sclerotic rabbits. Conclusion It suggests that high expression of OPN in the artery might be closely correlated with the development of atherosclerosis. Alltransretinoic acid may play an important role in inhibiting the development of atherosclerosis by decreasing the expression of OPN.
出处
《安徽医科大学学报》
CAS
北大核心
2009年第6期673-676,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:30971226)
安徽省自然科学基金(编号:090413091)
南京军区科技创新经费资助项目(编号:07M034-A)
解放军105医院科研课题基金(编号:2007001-A)