胚胎皮肤细胞裸鼠皮下移植构建毛发发育模型

Subcutaneous implantation of embryonic skin cells to construct a model of hair development in nude mice

目的观察胚胎皮肤细胞裸鼠皮下移植后的毛发发育情况,构建毛发发育模型。方法从C57BL/6鼠胚胎的皮肤中分离出真皮和表皮细胞,将它们按照一定比例重新混合后采用注射法移植到裸鼠皮下,显微镜下观察毛发形成情况。结果镜下观察到裸鼠皮下有毛囊及毛发纤维形成并有毛干长出。结论胚胎皮肤细胞体内移植可以构建毛发发育的完整模型。

Objective To observe the hair development after subcutaneous implantation of embryonic skin cells in nude mice, and construct a model of hair development. Methods Dermal and epidermal cells isolated from embryonic mouse skin were mixed at a given ratio and injected subcutaneously in nude mice, and hair formation after the implantation was observed under a microscope. Results Formation of the hair follicles and fibers under the skin of the recipient nude mice and emergence of the hair shaft were observed microscopically. Conclusion Embryonic skin cells be used to construct a complete model of hair development after implantation in vivo.