吸烟与温石棉单独及联合作用对人胚肺细胞蛋白激酶C活性的影响

Combined effect of cigarette smoke and chrysotile on the activity of protein kinase C in human embryo lung cells

为探讨细胞信号转导系统在吸烟与石棉联合致癌过程中的作用，本研究采用体外细胞培养技术，对香烟烟雾溶液（ＣＳＳ）和温石棉（ＣＨ）单独及联合作用于人胚肺（ＨＥＬ）细胞后细胞内蛋白激酶Ｃ（ＰＫＣ）活性的变化进行了观察。结果表明，ＣＳＳ与ＣＨ单独作用均可使胞浆ＰＫＣ（ＰＫＣ－Ｃ）和胞膜ＰＫＣ（ＰＫＣ－Ｍ）活性增强；当二者联合作用时可协同增强ＰＫＣ－Ｃ和ＰＫＣ－Ｍ的活性，提示吸烟与温石棉可能通过改变细胞内ＰＫＣ的活性进一步促进肿瘤的发生。

The activity of protein kinase C(PKC) in human embryo lung(HEL) cells were determined when the cells were exposed to cigarette smoke absorbed solution(CSS) and chrysotile(CH) separately or simultaneously. The results showed that both PKC in membrane(PKC M) and PKC in cytosol(PKC C) increased significantly when HEL cells were exposed to CSS and CH separately and increased synergisticaly when HEL cells were exposed to CSS and CH simultaneously. This result suggested that elevation of PKC activity in HEL cells induced by CSS and CH may play an importamt role in the promoting stage of carcinogenesis. \ \