三七总皂苷对异烟肼和利福平合用所致小鼠肝损伤的保护作用

The protective effect of PNS on liver injury induced by Isoniazid and Rifampin co-administration in mice

目的:观察三七总皂苷(PNS)对异烟肼(INH)和利福平(RFP)合用所致小鼠肝损伤的保护作用。方法:小鼠每天同时灌胃给予利福平和异烟肼各100mg/kg,2h后灌胃给予三七总皂苷100、200、400mg/kg,每天1次,连续给药10d,末次给药16h后,取血和肝脏,测定肝脏指数、血清谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性。结果:三七总皂苷能显著降低利福平和异烟肼合用引起的肝指数,并降低血清ALT和AST水平。结论:三七总皂苷对利福平和异烟肼合用所致小鼠肝损伤具有明显的保护作用。

Objective： To investigate the protective effect of PNS on liver injury induced by Isoniazid （INH） and Rifampin （RFP） co-administration in mice. Methods： Mice were simultaneously administrated orally with 100 mg/kg INH and RFP respectively. Two hours later, 100, 200, 400 mg/kg PNS were orally administered once a day for 10 days. After 16 hours of the last dosing, serum and liver were taken. Liver index, the level of ALT and AST in serum were determined. Results： PNS significantly decreased the liver index as well as the level of ALT and AST in serum. Conclusion： PNS has a protective effect on hepatic injury elicited by RFP and INH in mice.