肾康2号对HPA轴抑制模型大鼠的HPA轴影响的实验研究

The suppressing effect of Shenkang 2 on HPA axis of rats model

目的通过观察补肾健脾中药肾康2号对HPA轴抑制模型大鼠HPA轴的影响，为其在肾病综合征激素撤减、维持治疗阶段的临床应用提供理沧依据。方法以大剂量糖皮质激素制作大鼠HPA轴抑制模型，在激素撤减至小剂量维持时予肾康2号中药灌胃，观察治疗前后。肾康2号治疗组、激素对照组及正常组血清促。肾上腺皮质激素（ACTH）、糖皮质激素水平和肾上腺指数、肾上腺病理的差异。结果在激素撤减后，治疗组、对照组大鼠血清ACTH、糖皮质激素和肾上腺指数与治疗前比较均有显著回升；治疗组血清ACTH、糖皮质激素和肾上腺指数回升速度快于对照组；治疗组’肾上腺皮质束状带萎缩及细胞排列紊乱情况轻于对照组。结论肾康2号中药有改善糖皮质激素引起的HPA轴抑制作用。

Objective To observe the inhibition effect of Shenkang 2 on rats HPA axis, and pro- vide theoretical basis for clinical application of steroid withdrawal and maintenance in the treatment phase. Methods To built the HPA axis suppression rat model by using high-dose glucocorticoids, when the hormone reduced to low-dose, traditional Chinese medicine Shenkang 2 were administrated. Serum ACTH, cortisol levels, adrenal index, adrenal pathological differences were observed before and after the treatment of Shenkang 2 among the treatment group, hormone control group and normal group. Results Serum ACTH, cortisol and adrenal index slowly recovered after hormone withdrawal in both treatment group and the hormone control group, serum ACTH, cortisol and adrenal index in the treatment group rebounded faster than that in the control group, the atrophy of fascicular zone of adrenal cortex and cell disarrangement was lighter in the treatment group than that in the control group. Conclusions Shenkang 2 can improve the suppression effect of glucocorticoid-induced HPA axis suppression.