多发性硬化、视神经脊髓炎及临床孤立综合征患者脑脊液αB晶状体蛋白表达

αB-crystallin in Cerebrospinal Fluid of Patients with Multiple Sclerosis, Neuromyelitis Optica and Clinically Isolated Syndrome

目的探讨αB晶状体蛋白在多发性硬化（MS）、视神经脊髓炎（NMO）、临床孤立综合征（CIS）患者脑脊液中的表达，及其与MS临床分型的关系。方法对58例MS、NMO、CIS患者及21例对照组进行研究，MS分为传统MS（CMS）与视神经脊髓型MS（OSMS）。Western—blot法检测患者及对照组脑脊液αB晶状体蛋白。结果6例NMO、29例MS、23例CIS患者及21例对照组中，除2例CIS患者及1例对照组外，均可检测到分子量接近22Kda的条带，各组条带灰度强弱无显著差异，OSMS与CMS患者之间条带灰度强弱也无显著差异，各组间αB晶状体蛋白出现率的差异无统计学意义（P〉0．05）。结论αB晶状体蛋白在NMO、MS、CIS患者及对照组脑脊液中均存在，各组含量无显著差异，与MS临床分型无明显关系，αB晶状体蛋白在MS及NMO中的致病作用有待进一步证实。

Objective To investigate the existence of ctB-erystallin in eerebrospinal fluid （CSF）of patients with multiple sclerosis （MS） , neuromyelitis optica （ NMO ） and clinically isolated syndrome （CIS） , and its association to clinical typing of MS. Methods 58 subjects （29 MS,6 NMO and 23 CIS） and 21 controls were investigated in the study. MS patients were divided into opticospinal form of multiple sclerosis（OSMS） and conventional multiple sclerosis （CMS）. αB-erystallin in CSF was detected by Western-blot analysis. Results One band, which was corresponding to the molecular weight of 22KDa,was visualized in CSF of NMO, MS, CIS patients and controls ,without significant differences of grey scale. No significant differences of grey scale were seen be- tween OSMS and CMS patients either. The incidence of the band among NMO, MS, CIS patients and controls had no significant differences （ P 〉 0. 05 ）. Conclusion αB-crystallin exists in CSF of NMO, MS, CIS patients and controls, without significant differences among groups. And it has no association to clinical typing of MS. The pathogenic mechanism of αB-crystallin in MS and NMO needs to be further studied.