非程控降温、-80℃冻存自体外周血干细胞的研究

A STUDY ABOUT THE EFFECT OF A SIMPLIFIED METHOD FOR CRYOPRESERVATION ON PERIPHERAL BLOOD STEM CELLS

目的：观察非程控降温、－80℃冻存的方法对自体外周血十细胞（APBSC）的保存效果。方法：以6％羟乙基淀粉（HES）、5％二甲基亚砜（DMSO）及4％人血白蛋白（ALB）的混合物为冷冻防护剂，将APBSC直接置于－80℃下保存，冻存前反复苏后测定APBSC的CFU－GM、BFU－E；观察移植后造血功能重建情况。结果：13例患者白细胞在十3～＋7天下降至（0．0～0．1）×10／L，白细胞（0．0～0．2）×109／L持续时间3～6天，于＋9～＋11天恢复至1．0X109／L以上．中性粒细胞绝对值（ANC）于＋9～＋11天达到0．5X109／L。血小板在＋3～＋7天下降至（2．0～21）×109／L，于＋8～＋15天恢复至20×109／L以上。CFU－GM、BFU－E回大率分别为76．5％、78．4％。结论：非程控降温、－80℃冻存是一简便、经济、有效的自体外周血于细胞保存方法。

Objective: To observe the effect of cryopreservation on peripheral blood stem cells (PBSC) at -80℃ without rate-controlled freezing' Methods:PBSC were cryopreserved at -80℃ using 6% Hydroxyethyl starch (HES), 5 % DMSO and 4% albumin(ALB) as a cryoprotective agent without rate-controlled freezing' We examined the recovery rates of CFU-GM and BFU-E sample tubes cryopreserved by the simplified method' The speed of hematopoietic reconstitution was evaluated. Results:Thirteen patients reccived autologous PBSC transplantation' WBC reached 1.0X109/L at day + 9~ +11' The ANC rcached 0. 5 X 109/L at day +9~ + 11' The platelet count reached 20 X 109/L at day + 8^+15- Patients received 2~3 platelet transfusions. The recovery rates of CFU-GM and BFU-E were 76。5% and 78. 4%, respectively. Conclusion: The simplified method for cryopreservation is an economical and effective method for APBSC cryopreservation.