乌司他丁对大鼠全肝缺血-再灌注肺损伤的保护作用

Protective effect of ulinastatin on total hepatic ischemia-reperfusion lung injury in rats

目的观察乌司他丁对大鼠全肝缺血-再灌注(I-R)肺损伤的保护作用。方法24只SD大鼠随机分为全肝缺血-再灌注组(I-R组)、乌司他丁组(U组)及假手术对照组(C组),每组8只。检测支气管肺泡灌洗液(BALF)中总蛋白含量、肺组织干/湿质量比(D/W)、肺组织髓过氧化物酶(MPO)及超氧化物歧化酶(SOD)含量,并以逆转录聚合酶链式反应(RT-PCR)方法检测三组大鼠肺组织基质金属蛋白酶(MMP)14 mRNA的相对表达量。结果与U组和C组比较,I-R组大鼠肺组织BALF总蛋白和MPO含量明显增高,肺组织D/W和SOD显著降低,MMP14 mRNA相对表达量显著增高(P<0.05)。结论乌司他丁可减轻全肝I-R肺损伤;抑制肺组织MMP14 mRNA的表达可能是其机制之一。

Objective To study the protective effect of ulinastatin on total hepatic ischemiareperfusion（I-R）lung injury in rats. Methods Twenty-four male SD rats were randomly divided into three groups with 8 rats each. The rats in group C served as sham injury control,those in group I-R underwent 60 min reperfusion after 30 min total hepatic ischemia by clamping the hepatic hilum. The rats in group U were given ulinastatin 5 × 10^4 U/kg at 10 min just before the hepatic hilum occlusion, which was followed by I-R as in group I-R. The rats were sacrificed at end point of the operation and lung tissues were divided into several parts for pathological section, pulmonary MPO and SOD activity examination, and detecting MMPI 4 mRNA with RT-PCR. The total amount protein in bronchoalveolar lavage fluid（BALFP）and lung dry to wet （D/W）weight ratio were determined as welt. Results Compared to groups C and U, the total amount protein in BALFP and MPO in lung tissues were higher, lung D/W ratio and SOD were lower, and MMP14 mRNA in lung tissues was higher significantly in group I-R（P〈0.05）. Conclusion Severe acute lung injury after total hepatic I-R in rats is attenuated by ulinastatin. An inhibition of MMP14 mRNA expression in lung tissues may be one of the mechanisms for that.