舒芬太尼预先给药对大鼠急性局灶性脑缺血-再灌注损伤的保护作用

Sufentanil pre-dosage induces protection against acute focal cerebral ischemia-reperfusion injury in rats

目的观察舒芬太尼预先给药对大鼠大脑中动脉栓塞(MCAO)所致局灶性脑缺血-再灌注损伤的保护作用。方法40只雄性大鼠随机均分为四组,分别于缺血造模前腹腔注射舒芬太尼3μg/kg(S1组)、6μg/kg(S2组)、9μg/kg(S3组)和等容积生理盐水为对照组(C组)。给药后30 min,所有动物用右侧颈内动脉尼龙线线栓法致MCAO,120 min后恢复再灌注。再灌注24 h后记录神经功能障碍评分(NDS)。随后取大脑切片行TTC染色,计算脑梗死容积百分比。结果再灌注后24h,S1组NDS评分明显高于其他三组(P<0.05),梗死容积百分比明显低于其他三组(P<0.05),S2、S3、C组间NDS评分和梗死容积百分比差异均无统计学意义。结论舒芬太尼3μg/kg预先给药可明显减轻大鼠局灶性脑缺血-再灌注损伤。

Objective To investigate whether sufentanil pre-dosage induces protection aganist acute focal cerebral ischemia-reperfusion injury in rats. Methods Forty male SD rats were randomly divided into four groups with 10 rats each. Before establishing animal model, the rats were intraperitoneally injected sufentanil 3 μg/kg（group S1 ）,6μg/kg（group 82 ）, 9 μg/kg（group S2 ）or normal saline（group C）. At 30 min after injection, all rats subjected to the right middle cerebral artery oeclusion（MCAO） for 120 min,which was followed by reperfusion. The neurological deficit scores（NDS）were evaluated at 24 h after reperfusion. Infarct volume, as a percentage of volume at normal cerebral hemisphere, was determined by TTC staining. Results At 24 h after reperfusion, the NDS was higher and infarct volume was less in group 81 than those in groups of 82 ,Sa and C（P〈0. 05）. There were no significant differences in NDS and infarct volume among groups of S2, S3 and C. Conclusion Sufentanil preconditioning can protect rat brain against ischemiareperfusion injury.