酒精性肝硬化麦氏小体形态特征

STUDY OF MORPHOLOGICAL CHARACTERISTICS OF MALLORY BODY IN ALCOHOLIC LIVER CIRRHOSIS

目的：观察麦氏小体的形态特征及显示方法，探讨其在酒精性肝硬化中的阳性率。方法：应用酒精性肝硬化及慢性肝炎肝硬化尸检肝组织，经１０％福尔马林固定，石蜡包埋，ＨＥ染色和泛素免疫组化染色，观察麦氏小体形态。结果：麦氏小体在肝细胞胞浆内呈形态不规则、大小不一、密度不均的嗜伊红团块，在酒精性肝硬化之肝组织中，检出率１００％，而在肝炎肝硬化之肝组织中未见到。泛素免疫组化染色可清晰显示不同形态、大小麦氏小体，因而检出数量明显多于ＨＥ染色。结论：麦氏小体是酒精性肝病的形态特征之一，应用抗泛素单抗能清晰显示麦氏小体。

Objective:To study the morphology of Mallory body with its expression in alcoholic liver cirrhosis.Methods:Liver tissue obtained at autopsy was fixed with 10% formalin,embedded with paraffin and sectioned,then proceeded to HE stain and SP method immunohistostain.Results:Mallory body was seen as an intracytoplasmic eosinphilic structure,irregular in shape,in size and in density.It was found mainly in liver tissue of alcoholic cirrhosis with a positive rate of 100%(15/15),whereas there was no expression in those of post hepatitis cirrhosis(0/15).Much more Mallory bodies at various stages were detected by ubiquitin monoclone antibody labelling than that by HE stain.Conclusion:Mallory body is one of the main morphological characteristics of alcoholic liver cirrhosis,which could be used as an important indicative marker.Ubiquitin antibody labelling was proved to be a reliable and sensitive way of studying Mallory body.