老年人牙周炎龈组织内T和B淋巴细胞免疫组织化学研究

Immunohistochemical study of T and B lymphocytes in gingiva of periodontitis in old patients

目的：观察老年人牙周炎龈组织Ｔ和Ｂ淋巴细胞免疫组织化学变化。方法：对１５例老年牙周炎患者龈组织进行常规组织病理学检查，同时采用ＡＢＣ法对ＩｇＡ、ＩｇＧ、ＩｇＭ、λ、κ、ＣＤ４和ＣＤ８阳性细胞进行免疫组织化学染色观察，计算ＣＤ４：ＣＤ８的比值。结果：ＩｇＭ阳性细胞阳性率明显高于ＩｇＡ阳性细胞阳性率（Ｐ＜０．０５），ＣＤ４：ＣＤ８的比值降低。结论：老年人牙周炎龈组织免疫功能可能降低。

Aim: To observe immunohistochemical characteristics of T and B lymphocytes in old periodontitis patients. Methods: Gingiva tissues from 15 cases of old periodontitis patients had been stained histologically and had been labelled immunohistochemically with monoclonal antibodies for IgA, IgG, IgM, κ,λ,T4(CD4), T8(CD8). Then we calculated T4:T8 lymphocyte ratio. Results: In old periodontitis patients, the positive cases of IgM were more than those of IgA significantly.(P<0.05). T4:T8 lymphocyte ratio decreased significantly. Conclusions: Immunological function in gingiva tissue of old periodontitis patients may be low. Its histological and immunohistochemical characteristics are different from periodontitis patients of other aged groups.