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秀丽小杆线虫Caenorhabditis eleganspkc-2基因cDNA的克隆和表达

Cloning and expression pattern analysis of a cDNA of pkc-2 gene in Caenorhabditis elegans
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摘要 蛋白激酶C在秀丽小杆线虫中具有调节肌细胞渐进性萎缩的功能.为了揭示它的调节机制,本研究克隆了秀丽小杆线虫中蛋白激酶Cpkc-2基因的cDNApkc-2-c,构建了含该pkc-2基因cDNA亚型的重组质粒pPD118.20-pKG63;揭示了该cDNA在秀丽小杆线虫体壁肌细胞中的定位. Protein kinase C regulates the progressive muscle degeneration in Caenorhabditis elegans. In order to investigate the function of PKC involved in muscle degeneration, this paper cloned a cDNA isoform of pkc-2 gene of C. elegans and constructed the recombinant plasmids pPD118. 20-pKG63 containing the isoform. The new isoform was then further studied for gene expression pattern. Immunocytochemistry experiment showed that this eDNA isoform expressed in body-wall muscle cells and located near the dense body.
作者 钱雨 Laurent SEGALAT
机构地区 华东师范大学生命科学学院 克洛德·贝纳尔-里昂第一大学细胞与分子遗传学中心
出处 《华东师范大学学报（自然科学版）》 CAS CSCD 北大核心 2009年第6期134-140,共7页 Journal of East China Normal University(Natural Science)
基金 教育部-国家留学基金管理委员会2007年国家建设高水平大学公派研究生项目
关键词 秀丽小杆线虫 pkc-2基因 CDNA克隆 免疫细胞化学 Caenorhabditis elegans pkc-2 gene clone immunoeytochemistry
分类号 Q959.4 [生物学—动物学] X833 [环境科学与工程—环境工程]
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华东师范大学学报（自然科学版）
2009年 第6期

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