富含多糖多酚芒果果肉组织总RNA的提取

Isolation of total RNA from mango pulp tissue rich in polysaccharides and polyphenols

为寻求从芒果果肉组织提取总RNA的方法,以紫花芒果实为试材,在Tris-硼酸法基础上,在裂解液中加入聚乙烯吡咯烷酮除去干扰物质,在提取缓冲液中分别加入预冷的LiCl、异丙醇、无水乙醇沉淀剂,沉淀总RNA,结果表明,以异丙醇为沉淀剂的效果最理想,提取总RNA的获得率高,质量好,条带完整.该方法所提取的总RNA可用于RT-PCR、Northern杂交、cDNA文库构建等后续分子生物学研究.

The fruit of Zihua mango （Mangifera indica L.） was used as experimental material to improve the method for isolation of total RNA from mango pulp. This method, based on the modified Tirs-HaBO4 method, was improved by adding polyvinylpyrrolidone into the extraction solution, and the pre-cooling LiCl, isopropanol and ethanol absolute into the precipitation buffer. The results showed that total RNA precipitated by isopropanol was better than the other precipitants. Total RNA isolated by this method was of high quality with high ratio of yield and a full strip, and was able to be used for later molecular manipulations such as RT-PCR, Northen hybrid and cDNA library construction.