活性污泥驯化过程中芘降解菌的群落多样性分析

Community diversity analysis of pyrene degradable bacteria in the cultivable process of activated sludge

将采自炼油厂污水处理生化反应池的活性污泥以芘为唯一碳源进行富集培养与驯化,通过对6个不同时期混合菌群总DNA的提取,采用降落式PCR和变性梯度凝胶电泳(DGGE)技术对细菌16SrDNA基因V3区进行扩增和产物分离,对活性污泥驯化过程中芘降解菌的群落多样性进行了研究.结果表明,在驯化过程中,芘降解菌的群落结构和种群数量存在明显的演替过程,芘浓度的大小是影响芘降解菌的群落结构和种群数量的重要因素.

The refinery-activated sludge was cultivated with pyrene as one single source of carbon and energy. The community diversity of pyrene degradating strains was studied by extracting the genomic DNA of microbial community in 6 different periods, amplifying the bacterial 16S rDNA V3 fragment with touch-down PCR method, and separating the products by denaturing gradient gel electrophoresis （DGGE）. The result showed that there was an obvious syllogistic process in community structure and amount of dominant populations. The concentration of pyrene was a main factor to influence the community diversity ofpyrene degradable strains.