14-3-3σ蛋白在子宫肌瘤中的表达及意义

Expression and clinical significance of 14-3-3σ protein in uterine leiomyoma

目的:探讨14-3-3σ蛋白在子宫肌瘤中的表达及其临床意义。方法:采用RT-PCR及Western blot法分别对20例子宫肌瘤患者的肌瘤组织和同源正常肌层组织中的14-3-3σ表达情况进行半定量检测。结果:在20例子宫肌瘤患者的子宫肌瘤组织和同源正常肌层组织中,RT-PCR及Western blot法均检测到14-3-3σ基因表达,子宫肌瘤组织中出现明显的低表达或表达缺失。结论:14-3-3σ蛋白的低表达与子宫肌瘤的发生相关。

Objective： To explore the expression and clinical significance of 14-3-3σ protein in uterine leiomyoma. Methods： The expression levels of 14-3-3σ protein in uterine leiomyoma tissues and homologous normal myometrium tissues of 20 cases with uterine leiomyoma were measured by reverse transcription -polymerase chain reaction （RT -PCR） and Western blot. Results： RT- PCR and western blot both revealed that 14-3-3σ showed a basic expression in homologous normal myometrium group. The expression of 14-3-3σ in uterine leiomyomas tissues was low. Conclusion： The low level of 14-3-3σ protein is closely related to the pathogenesis of human uterine leiomyoma.