NSCLC组织中β-tubulinⅢ、stathm in蛋白的表达变化及其与紫杉醇敏感性的关系

Expression of stathmin and β-tubulinⅢ in NSCLC and its influence on taxol sensitivity

目的检测非小细胞肺癌（NSCLC）组织中的stathmin、β-tubulinⅢ,并探讨其与紫杉醇化疗疗效的关系。方法采用免疫组化SP法检测123例NSCLC患者肿瘤组织（观察组）和15例炎性假瘤患者瘤组织（对照组）中的stathmin、β-tubulinⅢ,并对其中32例根治术后用紫杉醇类药物行辅助化疗的疗效进行分析。结果NSCLC组织中stathmin、β-tubulinⅢ蛋白表达阳性率分别为52.8%、62.6%,显著高于良性对照组的6.7%、13.3%（P均〈0.05）。stathmin蛋白均在低分化和有淋巴转移的肿瘤中表达明显升高（P均〈0.05）;β-tubulinⅢ蛋白在低分化和Ⅲ-Ⅳ期肿瘤中表达均明显升高（P均〈0.05）。32例术后用含紫杉醇化疗方案化疗的患者中,stathmin、β-tubulinⅢ蛋白表达全阳性者的生存率较全阴性者低（P〈0.05）。结论NSCLC患者肿瘤组织中stathmin、β-tubulinⅢ蛋白均明显升高。二者表达均阳性预示着肿瘤的恶性程度较高,对紫杉醇类化疗药物的敏感性较差,术后预后较差。

Objective To observe the expression of stathmin and β-tubulinⅢ in non-small cell lung cancer（NSCLC）,and investigate its its influence on taxol sensitivity.Method Expression of stathmin andβ-tubulinⅢ in 123 NSCLC tissues（observation guoup）and 15 adjacent tissues（cotrol gyoup） were detected by immunohistochemical SP method.The taxol effect was analyzied in 32 patients who received taxol.Result The positive rates of stathmin,β-tubulinⅢ in observation guoup were 52.8%,62.6%;and 6.7%,13.3% in control group（P 〈 0.05）. The positive rates of stathmin have significant difference in poorly differentiated group and lymphatic metastasis group （ P 〈 0.05 ） ; The positive rates of β-tubulin Ⅲ have significant difference in poorly differentiated group and Ⅲ-Ⅳ state group （P 〈 0.05 ）. The expression of stathmin, β- tubulin Ⅲ in the patients who had postoperative chemotherapy were subdix ided into two group （all positive, all negative）, there showd significant survival difference between all positive and all negative. Conclusion The expression of stathmin , β-tubulin Ⅲ raise in NSCLC tissue. The tumor which stathmin , β-tubulin Ⅲ were both positive was more malignant, more insensitive to taxol has bad prognosisand.