肺癌组织中OATP-B、D蛋白及其mRNA表达变化及意义

Expression and significance of OATP-B,D protein and mRNA in lung cancer

目的检测肺癌组织中人类有机阴离子转运多肽(OATP)-B、D mRNA及其蛋白的表达,并探讨其意义。方法取40例肺癌组织(观察组)和10例正常组织(对照组),采用荧光定量RT-PCR法检测OATP-B、D mRNA,采用免疫组化法检测OATP-B、D蛋白。结果与对照组相比,观察组OATP-B和OATP-D的mRNA及蛋白表达均上调,P均<0.05。观察组有、无淋巴结转移者OATP-B、D mRNA及蛋白表达相比P均<0.05。结论肺癌组织中OATP-B、D mRNA及蛋白表达水平均升高。肺癌组织中OATP-B、D mRNA及蛋白表达,与淋巴结转移相关。

Objective To investigate the expression and clinical significance of organic anion transporting polypeptide （OATP） -B,D mRNA and protein in lung cancer. Methods The expressions of OATP-B and OATP-D mRNA were detec- ted in 40 eases of lung cancer and 10 cases normal lung tissues by SYBRGreen Quantitative Real-time PCR,as well as the expressions of OATP-B and OATP-D were available by immunohistochemistry analysis. Results The expressions of OATP- B and OATP-D mRNA were all significantly up-regulated in different pathological category lung neoplasms compared to normal lung tissues. There was also a significant difference in the expressions of OATP-B and OATP-D mRNA between lymph metastasis lung neoplasms and those without lymph metastasis, P 〈 0.05. Immunohistochemistry analysis showed a higher expression of OATP-B and OATP-D protein in lung cancers than normal lung tissues, as well as in lung cancers with lymph metastasis compared to those without lymph metastasis （P 〈 0.05）. Conclusion The expression of OATP-B and OATP-D mRNA and protein raise in lung cancer. Both of them are associated with lymph metastasis.