侧脑室微量注射apelin对大鼠血浆及脑内cAMP水平的影响

Effects on cyclic adenosine monophosphate content after micro-injecting apelin into lateral cerebral ventricles

目的观察侧脑室注射apelin对大鼠血浆及脑内cAMP含量的影响。方法侧脑室注射apelin,30 min后取血取脑,使用放射免疫法测定大鼠血浆、尾核及海马内cAMP的含量。结果与NS对照组相比,侧脑室注射apelin能明显增加大鼠血浆、尾核及海马内cAMP的含量。结论Apelin的这种痛觉敏感化作用至少部分是通过尾核、海马的调控作用而实现的,并且可能与细胞内apelin-cAMP信号转导途径相关。

Objective： To study the effect on cyclic adenosine monophosphate content in plasma and brain of rats after micro-injection of apelin into lateral cerebral ventricles. Methods： Brain and blood samples were taken from the decapitated rats at 30rain ＇after micro-injection of NS or apelin into lateral cerebral ventricles. The levels of cAMP in plasma, eaudate nucleus and hippocampus were measured by radioimmunoassay（ RIA ）. Results： The cAMP levels in plasma, caudate nucleus and hippocampus were increased significantly after injecting apelin （P 〈 0. 01 }. Conclusion： The hyperalgesica effect may be associated with caudate nucleus and hippocampus. The mechanism may be associated with an apelin-cAMP metabolic pathway.