益气活血方对高脂大鼠颈总动脉球囊损伤后血管内膜增生的影响

Effect of Yiqi Huoxue Prescriptions on Prevention of Neointima Hyperplasia after Carotid Artery Balloon Injury in Rats

目的观察益气活血方对高脂大鼠颈总动脉球囊损伤后血管内膜增生的抑制作用。方法将90只雄性SD大鼠随机分为模型组、阿司匹林组及益气活血方大、中、小剂量组。大鼠给予高脂饲料喂养30 d,造成高脂血症;然后经右股动脉穿刺球囊扩张损伤大鼠左颈总动脉,造成颈总动脉管腔的狭窄。手术前1 d开始给药,每组动物于术后5、14、28 d处死,取损伤颈总动脉,常规固定、包埋、切片,分别做HE染色。结果术后5 d血管狭窄不明显,术后14 d和28 d血管内膜明显增厚,管腔狭窄,模型组尤为明显。益气活血方干预后,大剂量组内膜增生平均厚度,残余管腔面积/总管腔面积与模型组相比,P<0.01,其余各用药组与模型组相比,P<0.05,结论益气活血方可显著抑制高脂大鼠颈总动脉球囊扩张后所致的血管内膜增生,益气活血方大剂量作用更明显。

Objective To investigate the effect of Yiqi Huoxue prescriptions on prevention of neointima hyperplasia after carotid artery vascular injury in rats. Methods Ninty male SD rats were randomly divided into 6 groups： the model group, the aspirin group, the low-dose Yiqi Huoxue group, the mid-dose Yiqi Huoxue group and the high-dose Yiqi Huoxue group. All of the rats were fed with hypercholesterolemic diet for 30 days, then the 2.0F balloon catheter injury was perfomed through the right femoral artery into the left common carotid artery of rats, the balloon was inflated and deflated 3 times. The rats was given drugs from i d before injury and to 5 d, 14 d, 28 d after injury. Around 6 rats from each group were euthanized by exsanguination at appropriate time point. The injuried left common carotid arteries were carefully removed and harvested for HE staining. Results The neointima hyperplasia was faint at 5 d after the operation. Morphological analysis demonstrated that there were markedly differences in the area of neointima hyperplasia at 14 d or 28 d, the average thickness of neointima hyperplasia, the surplus area of lumen/the total area of lumen between the high-dose Yiqi Huoxue group and the model group （P〈0.01）. Compared with the model group, those injury in drug groups showed obvious reduction （P〈0.05） at 14 d and 28 d after balloon. Conclusion Yiqi Huoxue prescriptions can effectively decrease neointima hyperplasia after balloon injury, the effect of the high-dose Yiqi Huoxue prescription group was remarkable.