益肾填精、补钙壮骨中药复方对骨形成蛋白-4诱导成骨信号转导机制的调控作用

Regulating Effect of Enriching Kidney Essence and Complementing Calcium to Strengthen Bone on BMP-4 Inducing Signal Transduction

目的研究益肾填精、补钙壮骨中药复方对骨形成蛋白-4(BMP-4)诱导成骨信号转导机制的调控作用。方法将雌性Wistar大鼠随机分为7组:正常组、模型空白组、牡蛎钙补肾中药复方低剂量组、牡蛎钙补肾中药复方中剂量组、牡蛎钙补肾中药复方高剂量组、骨疏康颗粒组、牡蛎碳酸钙咀嚼片组。采用切除大鼠双侧卵巢的方法复制绝经后骨质疏松症模型,应用益肾填精、补钙壮骨中药复方防治12周后进行取材及实验指标检测:用双能X线骨密度仪检测股骨骨密度;用逆转录多聚酶链反应(RT-PCR)检测大鼠骨组织BMP-4和Smad5、6 mRNA表达。结果与模型空白组比较,中药复方各剂量组股骨骨密度显著升高(P<0.01);骨组织BMP-4、Smad5 mRNA表达水平明显升高(P<0.01),Smad6 mRNA表达水平明显降低(P<0.01)。结论益肾填精、补钙壮骨中药复方可明显上调BMP-4和Smad5 mRNA表达,下调Smad6 mRNA表达。

Objective To study the regulating effect of complementing calcium and invigorating kidney method on the bone morphogenetic protein-4（BMP-4） and signal transduction mechanism.Methods Female Wistar rats were randomly divided into seven groups：normal group,model group,low-,middle-,high-dose yster calcium compound Chinese medicine for reinforcing kidney group,Gushukang granule group,oyster shell calcium tablets group.The model of postmenopausal osteoporosis was established through ovariectomy in rats.The osteoporosis rats were treated with nanometer calcium and invigorating kidney Chinese medicine Gushukang granule, and oyster shell carbonate chewable tablets were used as positive-control groups, the normal rats as standard control, and the model rats as model control. After 12 weeks of the treatment, the indexes were tested. The bone mineral density （BMD） of femoral bone in vitro was detected by dual-energy X-ray densitometer and the expression of BMP-4 and Smad 5, 6 mRNA were detected by reverse transcription-polymerase chain reation （RT-PCR）. Results Compared with the model rats, BMD of femoral bone in Chinese medicine groups （high dose, middle dose, low dose） increased obviously （P〈0.01）. The expression of BMP-4 and Smad5 mRNA increased obviously （P〈0.01）, and the expression of Smad6 mRNA decreased obviously （P 〈 0.01）. Conclusions Nanometer calcium and invigorating kidney Chinese medicine can increase the expression of BMP-4 and Smad5 mRNA, and decrease the expression of Smad6 mRNA.