摘要
目的观察莪术油注射液对人卵巢癌SKOV3细胞胀亡指数及Bcl-2表达的影响,探讨莪术油注射液致人卵巢癌SKOV3细胞胀亡的机制。方法以不同浓度莪术油注射液作用人卵巢癌SKOV3细胞后,通过荧光显微镜观察细胞核变化、透射电镜计算细胞胀亡指数、琼脂糖凝胶电泳观察细胞DNA断裂情况、免疫组化观察细胞Bcl-2基因的表达。结果人卵巢癌SKOV3细胞经莪术油注射液作用48 h后,荧光显微镜观察到胀亡细胞出现细胞肿胀,体积增大,胞膜面积缩小,核染色质分散扩大;细胞胀亡指数随浓度的增加而增大,呈量效关系;电泳观察DNA呈弥漫型;Bcl-2基因表达下调。结论莪术油注射液可使人卵巢癌SKOV3细胞随浓度的增加而胀亡指数增大,并可下调Bcl-2基因表达,提示下调Bcl-2基因表达可能是莪术油注射液致SKOV3细胞胀亡的作用机制之一。
Objective To discuss the impact of zedoary turmerie oil(ZTO) injection over oncosis index and Bcl-2 expression of human ovarian cancer SKOV3 cell.Methods After ZTO injection acted on human ovarian cancer SKOV3 cells,the changes in the nucleus were observed by fluorescence microscope,oncosis index was counted by projection electron microscope,the situation of cell DNA breakage was observed by agarose gel electrophoresis,and cell Bcl-2 gene expression was observed by immunohistochemical method.Results After treated by ZTO injection for 48 hours, human ovarian cancer SKOV3 cells shows that oncosis cell was swelling in fluorescence microscope, the volume increased, the membrane area narrowed, the nuclear chromatin expansed. The oncosis index increased with dose-effect relationship, DNA electrophoresis showed diffuse type and Bcl-2 gene expression was down-regulated. Conclusion ZTO injection can increase oncosis index of human ovarian cancer SKOV3 cell with the concentration, and lower Bcl-2 gene expression, which may be one of the mechanisms of ZTO injection caused oncosis of SKOV3 cell.
出处
《中国中医药信息杂志》
CAS
CSCD
2009年第10期34-35,80,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
2006年广西中医学院高校基金(ZD2006008)
广西高校人才小高地建设创新团队资助计划:桂教人[2005]34号