摘要
目的探讨脾虚型哮喘大鼠气道炎症,血液和支气管肺泡灌洗液(BALF)中嗜酸性粒细胞百分比,肺组织核因子-κB抑制蛋白α(IκBα)表达和核因子-κB(NF-κB)活性的变化及补脾益气方药对其的影响。方法将30只雄性Wistar大鼠随机分为3组:正常对照组(A组)、脾虚型哮喘组(B组)、补脾益气方药组(C组),采用HE染色检测肺组织病理变化,采用免疫组织化学方法检测肺组织IκBα表达水平和NF-κB p65的活性变化。结果B组与A组比较,肺组织IκBα表达显著降低,而NF-κB p65的活性显著增高(P<0.01);C组与B组比较,肺组织IκBα表达显著增高,而NF-κB p65的活性显著降低(P<0.01)。结论补脾益气方药能有效增加脾虚型哮喘大鼠肺组织IκBα的表达,抑制其肺组织NF-κBp65的活性,减轻哮喘时的气道炎症变化,从而对脾虚型哮喘起到治疗作用。
Objective To investigate airway inflammation,number of eosinophils in blood and BALF,the expression of inhibitor protein α(I κB α) and nuclear factor-κB(NF-κB) activity in lung of rat with spleen deficiency asthma,and the effect of herbs of reinforcing spleen to replenishing Qi.Method Thirty rats were randomly divided into three groups:control group(group A),spleen deficiency asthma group(group B),decoction group(group C).The pathological changes of lung was detected by HE staining,and the expression of I-κB a was significantly increased, and activity of NF-κB p65 was significantly lowered in group C (P 〈0.01). Conclusion Herbs of reinforcing spleen to replenishing qi can effectively played treatment of asthma in rats of spleen deficiency by increasing lung tissue I-κB a expression, inhibiting NF-κB p65 activity and reducing airway inflammation.
出处
《中国中医药信息杂志》
CAS
CSCD
2009年第10期36-38,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
辽宁科学技术基金(20072078)