慈菇消脂丸中大黄素和大黄酚含量的测定

Content Determination of Emodin and Chrysophanol in Cigu Xiaozhi Pill

目的采用高效液相色谱法测定慈菇消脂丸中大黄素、大黄酚的含量。方法采用Symmetry C18色谱柱(4.6 mm×150 mm,5.0μm),流动相为甲醇-0.1%磷酸溶液(85∶15),流速:0.8 mL/min;检测波长:423 nm;柱温:20℃。结果大黄素、大黄酚在0.25～0.75μg范围内与峰面积呈良好线性关系,平均加样回收率分别为98.0%(RSD=1.5%)、99.6%(RSD=1.8%)。结论本方法稳定、简便易行,为慈姑消脂丸的生产提供了质量依据。

Objective To establish a HPLC method for the determination of the content of emodin and chrysophanol in Cigu Xiaozhi pill.Methods The chromatographic procedure was carried out in Symmetry C18（4.6 mm×150 mm,5.0 μm） column with methanol-0.1% phosphoric acid（85∶15） as the mobile phase,flow rate was 0.8 mL/min,column temperature was 20 ？C,and the detection wavelength was 423 nm.Results Emodin and chrysophanol were well separated with other components.The standard curves of emodin and chrysophanol showed lineadty in the range of 0.25-0.75μg. The average recoveries were 101.35% （RSD=2.26%） and 98.66% {RSD= 1.74%） respectively. Conclusion The method is sensitive, simple and accurate, and can be used for the determination of emodin and chrysophanol in Cigu Xiaozhi pill.