美洲大蠊各虫态粪便提取物的聚集活性研究

Aggregation of extracts from different stage of Periplaneta americana faeces

目的研究美洲大蠊各虫态粪便的诱集活性,为简单有效地提取聚集信息素并应用于美洲大蠊的防治提供线索。方法用丙酮和乙醇混合溶剂分别提取美洲大蠊雌虫、雄虫及老熟幼虫粪便,测定提取物对美洲大蠊各虫态的诱集活性,数据采用SAS8.0软件进行分析。结果3种虫态粪便提取物对美洲大蠊各虫态都具有明显的诱集作用,其中老熟幼虫粪便提取物对老熟幼虫的引诱效果最好,引诱率达到59%;雄虫粪便提取物对老熟幼虫的引诱活性最高,引诱率为41%;雌虫粪便提取物对雄虫的引诱活性最好,引诱率也可以达到42%,且各种提取物对美洲大蠊老熟幼虫都有较高的聚集活性。结论美洲大蠊各虫态粪便中含有聚集信息素,且活性各不相同,可以进一步研究应用于美洲大蠊的防治。

Objective Study the attractant activity of faeces from different stage of Periplaneta americana and provide clues for the effective prevention of cockroach. Methods The extracts with attractant activity were extracted from female adult faeces, male adult faeces and larva faeces of P. americana by ethanol and acetone. The data was analyzed by software SAS 8.0. Results All the extracts attracted P. americana significantly. The attractant activity of extracts from high-instar nymphs faeces was the best, and the attractant rate reached 59%. The extracts from male faeces had strong attractant to high-instar nymphs with the attractant rate of 41%, and that from female faeces attracted more male adults than other development stages with the attractant rate of 42%. Three extracts had higher attractant to the larvae. Conclusion The faeces of P. americanav have aggregation pheromone which activity are different, and can do more research to control cockroach.