一种改进的抗人体蠕形螨药物筛选的实验方法

A modified drug screening method for evaluating in vitro anti-mite activity

目的寻找一种简便易行和定量的体外抗人体蠕形螨药物筛选的实验方法。方法以百部乙醇提取物为实验药物,分别应用文献记载的各种方法和自创的跟踪法进行体外抗人体蠕形螨实验,评价各种方法对筛选抗螨药物活性的价值。结果传统各法在药物和螨的作用方法及药物抗螨效果评价等方面均各有利弊。改进的跟踪法简单易行、视野清晰并可定位跟踪螨的变化,准确记录每只螨的死亡时间,用x±s表示螨的死亡时间为(3.53±1.04)min,可用t检验比较不同药物的抗螨活性。结论跟踪法是一种较为理想的进行体外抗人体蠕形螨药物活性筛选的实验方法。

Objective To develop a direct and effective method to screen drug that can kill human Demodex in vitro. Methods Several methods including self-established method named Tracking-Detection were used in anti-mite experiments in vitro with Radix Stemonae alcohol extract individually. Way of drug delivery and anti-mite effect of all methods were evaluated. Results All the traditional methods showed advantages and disadvantages in drug delivery and evaluation of anti-mite effect. Tracking-Detection was easy to be conducted with clear visual field, and the exact death time of each mite could be detected and the death time was （3.53 ± 1.04） rain with Radix stemonae alcohol extract. And then the differences of the anti-mite activities to human demodex of different drugs could be compared statistically by t-test. Conclusion Tracking-Detection is an ideal method to screen anti-mite drug killing human Demodex in vitro.