对肾综合征出血热传播媒介小盾纤恙螨的研究

Investigation of Leptotrombidium scutellare-hemorrhagic fever with renal syndrome vector

目的探索小盾纤恙螨在肾综合征出血热(HFRS)传播中的作用及传播疾病的关系。方法在疫区捕获野鼠,采集小盾纤恙螨,用Vero-E6细胞组织培养及间接免疫荧光法(IFA)检测汉坦病毒(HV)感染情况,并用RT-PCR检测螨体内HV-RNA,观察螨体内HV增殖,原位RT-PCR分子杂交法进行螨体内HV定位。结果IFA检测HV抗原阳性鼠寄生小盾纤恙螨HV自然感染率为66.7%,IFA检测阴性鼠寄生螨HV自然感染率为25.0%;该螨通过叮刺和经卵传播HV。结论小盾纤恙螨为野鼠型HFRS主要传播媒介之一,在传播HFRS中的作用不容忽视。

Objective Explore the role of chigger mite in the transmission of hemorrhagic fever with renal syndrome （HFRS）. Methods Chigger mites were collected from rats caught in the plague foei. HV was detected by Vero-E6 culture and IFA. HV-RNA from mites was tested by RT-PCR, and HV was located by hybridization histoehemistry. Results The infection rate of HV was 66.7% in chigger mites collected from rats which antigens were positive, however it was only 25.0% in mites from rats which antigens were negative. The result showed that chigger mite could transmit HFRS by stinging and vertical transmission. Conclusion It indicates that chigger mite is one of HFRS vectors, which roles in HFRS transmission can not be neglected.