二烯丙基二硫通过细胞外信号调节激酶途径调节HepG2细胞Survivin表达

Regulation of Survivin Expression by Diallyl Disulfide and ERK Pathway in HepG2 Cells

目的探讨二烯丙基二硫(DADS)对HepG2细胞增殖的影响及其机制。方法W estern b lot法检测survivin、细胞外信号调节激酶(ERK1/2)和p-ERK1/2的表达;AO/EB染色观察细胞形态学变化。结果ERK1/2抑制剂PD98059抑制DADS诱导survivin表达的作用。PD98059与DADS联合应用可以促进HepG2细胞凋亡。结论DADS诱导HepG2细胞survivin的表达与ERK1/2信号通路有关。

Objective To investigate the effect and the mechanism of of HepG2 cells proliferation by diallyl disulfide （ DADS ）. Methods The proteins expression of extracellular signal - regulated kinase （ ERK1/2 ）, p - ERK1/2 and survivin was detected by western - blot. Morphological assessment of apoptosis was by acridine orange （ AO ） and ethidium bromide （ EB ） fluorescence double staining. Results An ERK1/2 inhibitor PD98059 diminished the expression of survivin which was induced by DADS. PD98059 combining with DADS resulted in HepG2 cells apoptosis. Conclusions The ERK signaling pathway was involved in DADS -induced survivin expression.