蓖麻毒蛋白碘油乳剂介入治疗裸鼠肝癌的实验研究

An Experimental Study on the Therapeutic Effects of Ricin Intervention on Hepatoma

目的观察蓖麻蛋白碘化乳剂介入治疗肝癌的疗效，比较蓖麻蛋白碘化乳剂与丝裂霉素对骨髓抑制的差别。方法用人肝癌裸小鼠皮下移植瘤为组织来源，植入实验裸鼠肝内建立裸鼠肝癌模型，以瘤体内注入生理盐水作对照，比较蓖麻蛋白（４μｇ／ｋｇ）碘油乳化蓖麻蛋白（８μｇ／ｋｇ）丝裂霉素（５００μｇ／ｋｇ）（３种剂型用量均为半数致死量的三分之一）肝癌瘤体内注射后的疗效，观察肿瘤生长抑制率、甲胎、血像及骨髓改变。结果蓖麻蛋白、碘油乳化蓖麻蛋白、丝裂霉素组肿瘤生长受到显著抑制（Ｐ＜０．０５），肿瘤组织发生不同程度坏死，甲胎蛋白降低与对照组比较有显著差别（Ｐ＜０．０５），但３种剂型间无显著性差别。周围血像及骨髓抑制丝裂霉素比蓖麻蛋白明显（Ｐ＜０．０１）。结论蓖麻蛋白对肝癌的治疗作用明显，其乳剂抗癌作用不变，为蓖麻蛋白瘤内注射及乳化后肝动脉导向治疗提供了实验依据，蓖麻蛋白骨髓抑制毒性明显低于丝裂霉素，为反复给药提了条件。

Objective To observe the therapeutic effects of ricin intervention in the treatment of experimental hepatoma and to compare the restraint to marrow under ricin and mitomicim C therapy.Methods Man’s hepatoma being first transplanted subcutaneously into nude mouse was used as the origin of the tissue,which later retransplanted into the experimental nude mouse’s liver to set up a nude mouse hepatoma model.The therapeutic effect of ricin (4μg/kg) was compared with that of lipiodol emulsified ricin (8μg/kg),and mitomicim C (500μg/kg) (all these dosages are onethird of LD50) with saline as the control,all administered by injecting into the tumor body to observe the tumour growth inhibition rate,α FP,hemogram and the change of marrow change.Results The tumour growth of groups of ricin,lipiodol emulsified ricin,and mitomicim was inhibited respectively,resulting in tumour necrosis.The inhibition of marrow was showed more evidently in mitomicim C than that in ricin.The reduction of α FP was significant as compared with that of the control group,however,there is no remarkable differences among the three forms.Conclusion Ricin has remarkable therapeutic effect on hepatoma. Ricin’s emulsion didn’t make any change which provide experimental evidence that Rican might be injected into tumor and emulsified ricin might be induced into tumor through liver artery.The toxicity to marrow was obviously lower than mitoinicin C.This might offer opportunity to repeat medication.