菱角皮提取物中鞣酸类化合物的含量测定及其指纹图谱研究

Determination of Tannin Compounds in Extract from the Fruit Crust of Trapa bispinosa Roxb and its Fingerprint

目的建立菱角皮醋酸乙酯提取物中鞣质类化合物的HPLC含量测定方法,并对提取物HPLC指纹图谱条件进行研究分析。方法采用反相C18柱(4.6 mm×200 mm,5μm),甲醇-0.5%乙酸水溶液为流动相,梯度洗脱,柱温为25℃,流速为0.8 ml/min,在276 nm下进行检测。结果菱角皮醋酸乙酯提取物中鞣质类化合物1,2,6-三没食子酰-β-D-葡萄糖的含量为85.7 mg/g,加样回收率为92.49%;1,2,4,6-四没食子酰-β-D-葡萄糖的含量为244 mg/g,加样回收率为96.41%;初步探讨了菱角皮提取物的指纹图谱条件。结论采用高效液相法测定菱角皮醋酸乙酯提取物中鞣质类化合物的含量,简便、快速、准确;初步得到分离菱角皮提取物各特征峰的色谱条件,为以后建立指纹图谱和制定菱角皮药材及其活性提取物的质量标准提供了依据。

Objective To establish determination method of tannin compounds from bioactive ethyl acetate extract of the fruit crust of Trapa bispinosa Roxb by HPLC, and to study on analysis conditions of HPLC fingerprint of the extract. Methods Use reverse phase C18 column（4. 6 mm×200mm,5μm）, detection wave -length at 276 nm, column temperature at 25℃, flow rate at a 0. 8 ml/min, and gradient aqueous methanol solution containing 0.5% acetic acid as a mobile phase. Results The determination of tannic acid compound 1, 2, 6 - tri - galloyl - β - D - glucose from the extract was 85.7mg/g, and the average recoveries was 92.49% ; the determination of tannic acid compound 1,2, 3, 6 - tetra - galloyl - β- D - glucose was 244 mg/g, and the average recoveries was 96.41% ; a preliminary study on conditions of HPLC fingerprint for the bioactive extract was carried out. Conclusion The determination method for the tannin compounds from ethyl acetate extract of the fruit crust of the plant by HPLC is simple, fast and accurate. Chromatographic conditions of ethyl acetate extract of the plant was initially obtained, which provide the basis for establishment of the HPLC fingerprint and making quality standard of the crude drug and its bioactive extract from the plant.