Cu(Ⅱ)与HSA或BSA结合的研究

STUDIES ON THE BINDING BETWEEN Cu(Ⅱ) AND HSA OR BSA

用平衡透析法研究了生理条件（ｐＨ７．４３）下Ｃｕ（Ⅱ）与人血清白蛋白（ＨＳＡ）或牛血清白蛋白（ＢＳＡ）的相互作用．结果表明，ＨＳＡ对Ｃｕ（Ⅱ）有１个强结合部位和９个弱结合部位，结合常数分别为１１×１０５及９１×１０２；ＢＳＡ对Ｃｕ（Ⅱ）有１个强结合部位和７个弱结合部位，结合常数分别为８４×１０４和７５×１０２．

The binding of Cu(Ⅱ) with human serum albumin (HSA) or bovine serum albumin (BSA) at physiological pH(7.43) is studied by the method of equilibrium dialysis. The results show that there exists one strong binding site and nine weak binding sites of Cu(Ⅱ) in HSA. The stability constants are 1.1×10 5 and 9.1×10 2 respectively. In BSA there are one strong binding site and seven weak binding sites of Cu(Ⅱ). The stability constants are 8.4×10 4 and 7.5×10 2 respectively. The Hill coefficients demonstrate that the binding of HSA or BSA with Cu(Ⅱ) has some positive cooperativity.