大白菜DH_0代群体遗传多样性的两种分析方法

Two Analyzing Methods for Genetic Diversity of Population in DH_0 Generation of Chinese Cabbage

以游离小孢子培养获得的KF大白菜(丰抗70)DH0代群体和用于小孢子培养的F1代杂交亲本,共17份实验材料,采用系统聚类和主坐标分析,利用RAPD标记技术分析了其遗传稳定性。从106条RAPD随机引物中筛选出33条重复性好并且具有多态性的引物进行扩增,在17份材料中扩增了200条带,其中多态带为120条(60%)。通过对RAPD分析数据进行系统聚类与主坐标分析,2种分析结果大体一致,表明系统聚类与主坐标分析这2种方法对种质资源进行聚类分组都具有可行性。

The genetic stability of 16 populations in DH0 generation derived from isolated microspore culture and one population in F1 hybrid parent derived from microspore culture was studied by the technique of Random Amplified Polymorphic DNA （RAPD）. 33 repeated and polymorphic primers were selected from 106 random primers of RAPD to be amplified. 200 distinct bands were yielded from 17 samples, and there were 120 polymorphic bands among them （60%）. The analyzing results from two different clustering methods were generally identical, and it indicated the analyses of systemic clustering and principal coordinate were all feasible to cluster groups for germplasm resources.