高效液相色谱法测定化妆品中邻苯二甲酸酯

HPLC Determination of Phthalates in Cosmetics

采用高效液相色谱法测定化妆品中的邻苯二甲酸酯含量。样品用甲醇超声提取,高速离心,上清液经0.45 μm滤膜过滤后,SB-C_(18) ZORBAX色谱柱(4.6 mm×150 mm,5 μm)分离,甲醇和水为流动相梯度淋洗,在230 nm波长检测。邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二丁酯(DBP)、邻苯二甲酸二己酯(DHP)和邻苯二甲酸二辛酯(DOP)的峰面积与其质量浓度均在1～100 mg·L^(-1)范围内呈线性关系,方法的检出限(3S/N)分别为4.0,7.4,4.3,5.2,7.3 ng。5种邻苯二甲酸酯的加标回收率在94.7%～101.1%之间,相对标准偏差(n=6)在0.023%～0.68%之间。

HPLC was applied to the determination of phthalates, including dimethyl phthalate （DMP）, diethyl phthalate （DEP）, dibutyl phthalate （DBP）, dihexyl phthalate （DHP）, and dioctyl phthalate （DO）P） in cosmetics. The sample was ultrasonically extracted with methanol and centrifuged at high speed. The supernatant liquid obtained was filtrated with 0. 45 μm membrane, and was separated by gradient elution with methanol and water on SB-C18 ZORBAX column （4. 6 mm× 150 mm, 5 μm）. The contents of phthalates were determined at the wavelength 230 nm by external standard method. Linear relationships between the values of peak area and mass eoneentration of 5 phthalates were kept in the range of 1--100 mg·L-1, with detection limits （3S/N） of 4. 0, 7.4, 4. 3, 5. 2, and 7. 3 ng, respectively. The values of recovery found by standard addition method were in the range of 94. 7%-101.1%, with RSD＇s （n=6） ranged from 0. 023% to 0. 68%.