白腐真菌对造纸黑液中木质素的降解效果

Study on factors influencing the degradation of lignin in black liquor by white-rot fungus

【目的】应用白腐真菌处理造纸黑液,探讨白腐真菌对造纸黑液中木质素的降解效果及其影响因子,确定最佳降解条件。【方法】从自然界腐朽木材上分离培养白腐真菌,利用专用培养基对其进行分离和纯化,分析木质素溶液质量浓度(污染负荷)、pH值、搅拌速度、碳源和氮源质量浓度、[Fe2+]质量浓度对白腐真菌降解木质素效果的影响。【结果】确定白腐真菌降解造纸黑液中木质素的最佳条件为:木质素质量浓度为153.0 mg/L,pH值为5,搅拌速度为250 r/min,葡萄糖质量浓度为1 g/L,酒石酸铵质量浓度为0.20 g/L,[Fe2+]质量浓度为0.08 mg/L。【结论】得到了白腐真菌降解造纸黑液中木质素的最佳条件,在此条件下白腐真菌对木质素的降解率最高可达64.38%。

[Objective] The study chose the white-rot fungus to process black liquor to explore the degradability and the influence factors of white-rot fungus on lignin of black liquor. [Method] Special culture medium was used to separate and purify white-rot fungus which had been separated from the rotten wood and cultured. The influence of degradation was analyzed by the following ingredients.lignin consistency, pH value,stirring speed,consistency of carbon and nitrogen source, and Fe^2＋ consistency, so as to choose the best conditions of white-rot fungi for degradation. [Result] The best condition of degrading black liquor is determined：lignin= 153 mg/L, pH = 5, the rotating speed is 250 r/min, glucose = 1 g/L, ammonium tartr ate=0.20 g/L,Fe^2＋ =0.08 mg/L. [Conclusion] The best degradation conditions in which white-rot fungus degrade black liquor are obtained. Under these conditions, the degradation rate of white-rot fungus is up to 64.38%.